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nbd cholesterol 22 n 7 nitrobenz 2 oxa 1 3 diazol 4 yl amino 23 24 bisnor 5 cholen 3β ol  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nbd cholesterol 22 n 7 nitrobenz 2 oxa 1 3 diazol 4 yl amino 23 24 bisnor 5 cholen 3β ol
    Nbd Cholesterol 22 N 7 Nitrobenz 2 Oxa 1 3 Diazol 4 Yl Amino 23 24 Bisnor 5 Cholen 3β Ol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nitrobenz+2+oxa+1/Cholesterol/pm41965690-187-7-20
    Average 98 stars, based on 1 article reviews
    nbd cholesterol 22 n 7 nitrobenz 2 oxa 1 3 diazol 4 yl amino 23 24 bisnor 5 cholen 3β ol - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Dapagliflozin Reduces Apoptosis of Diabetic Retina and Human Retinal Microvascular Endothelial Cells Through ERK1/2/cPLA2/AA/ROS Pathway Independent of Hypoglycemic
    Article Snippet: .. In the first stage, HRMECs were incubated with different glucose concentrations (5.5 mmol/L, 30 mmol/L, 60 mmol/L) for 24 h. In the second stage, HRMECs were incubated with high glucose (30 mmol/L) with or without DAPA (1 μmol/L) for 24 h. In the third stage, HRMECs were incubated with different concentrations of 2-(N-(7-nitrobenz-2-oxa-1, 3-diazol -4-yl) amino)-2-deoxy-D-glucose (2-NBDG, Invitrogen, United States) (0, 50, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000 μmol/L) for 30 min or with 2-NBDG for different times (0, 5, 15, 30, 60, 120 min) with or without DAPA (1 μmol/L). ..

    Article Title: Crosstalk between Metabolic Alterations and Altered Redox Balance in PTC-Derived Cell Lines
    Article Snippet: .. Cells were seeded in 96-well plates (1 × 10 5 cells/mL) and incubated for 24 h. After incubation, plates were washed with PBS, followed by the addition of 50 μM of 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino]-2-deoxy-Dglucose (2-NBDG, fluorescently-tagged glucose derivative, N13195; Invitrogen), which was incubated for 30 min. ..

    FACS:

    Article Title: Suppression of autophagy by FIP200 deletion inhibits mammary tumorigenesis
    Article Snippet: Three weeks after seeding, colonies were stained with p-iodonitrotetrazolium violet (Sigma), and those >50 μm in diameter were counted under a microscope. .. Glucose uptake was measured by FACS detection of uptake of 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino]-2-deoxyglucose (2-NBDG; Invitrogen) in cultured cells as described previously ( Lock et al. 2011 ). .. Intracellular lactate level was measured using Lactate Assay Kit II (Biovision).

    Cell Culture:

    Article Title: Suppression of autophagy by FIP200 deletion inhibits mammary tumorigenesis
    Article Snippet: Three weeks after seeding, colonies were stained with p-iodonitrotetrazolium violet (Sigma), and those >50 μm in diameter were counted under a microscope. .. Glucose uptake was measured by FACS detection of uptake of 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino]-2-deoxyglucose (2-NBDG; Invitrogen) in cultured cells as described previously ( Lock et al. 2011 ). .. Intracellular lactate level was measured using Lactate Assay Kit II (Biovision).

    other:

    Article Title: A comparative evaluation of antioxidant and antidiabetic potential of peel from young and matured potato
    Article Snippet: In the present study antioxidant and antidiabetic potentials of potato peels at two different stages of maturity were evaluated and compared.. Peels of young and mature potatoes (YP and MP) were sequentially extracted with hexane (HMP, HYP), ethyl acetate (EMP, EYP) and methanol (MMP, MYP).. EMP and EYP were found to possess the highest phenolic content (83.2 and 44.14 mg GAE/g dry weight, respectively) and maximum radical scavenging efficacy for different antioxidant assays performed.



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    SYK deficiency impairs glycolytic flux in SCs. ( A ). ECAR profiles measured by Seahorse experiments. ( B ). Quantification of basal glycolysis, glycolytic capacity, and glycolytic reserve in ECAR. n = 4 independent biological experiments. ( C ). Glucose uptake measured <t>with</t> <t>2-NBDG</t> by flow cytometry. n = 4 independent biological experiments. ( D ). Intracellular levels of glucose-6-phosphate (G6P), pyruvate and lactate were measured by chemiluminescence. n = 4 independent biological experiments. ( E ). Protein levels of GLUT1, GLUT4, HK2, PFK-1, PFKFB3, PKM2 and LDHA in SCs were measured by western blot. n = 3 independent biological experiments. ( F ). Immunofluorescence staining and quantification of GLUT1, HK2, PFK-1, and PFKFB3 in SCs. Scale bar, 20 μm. N = 4 independent biological experiments, with n ≥ 6 fields/experiment. ( G ). Schematic depicting SYK deficiency-induced downregulation (red arrows) of glycolytic transporters, enzymes, and metabolites in SCs
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    SYK deficiency impairs glycolytic flux in SCs. ( A ). ECAR profiles measured by Seahorse experiments. ( B ). Quantification of basal glycolysis, glycolytic capacity, and glycolytic reserve in ECAR. n = 4 independent biological experiments. ( C ). Glucose uptake measured <t>with</t> <t>2-NBDG</t> by flow cytometry. n = 4 independent biological experiments. ( D ). Intracellular levels of glucose-6-phosphate (G6P), pyruvate and lactate were measured by chemiluminescence. n = 4 independent biological experiments. ( E ). Protein levels of GLUT1, GLUT4, HK2, PFK-1, PFKFB3, PKM2 and LDHA in SCs were measured by western blot. n = 3 independent biological experiments. ( F ). Immunofluorescence staining and quantification of GLUT1, HK2, PFK-1, and PFKFB3 in SCs. Scale bar, 20 μm. N = 4 independent biological experiments, with n ≥ 6 fields/experiment. ( G ). Schematic depicting SYK deficiency-induced downregulation (red arrows) of glycolytic transporters, enzymes, and metabolites in SCs
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    SYK deficiency impairs glycolytic flux in SCs. ( A ). ECAR profiles measured by Seahorse experiments. ( B ). Quantification of basal glycolysis, glycolytic capacity, and glycolytic reserve in ECAR. n = 4 independent biological experiments. ( C ). Glucose uptake measured <t>with</t> <t>2-NBDG</t> by flow cytometry. n = 4 independent biological experiments. ( D ). Intracellular levels of glucose-6-phosphate (G6P), pyruvate and lactate were measured by chemiluminescence. n = 4 independent biological experiments. ( E ). Protein levels of GLUT1, GLUT4, HK2, PFK-1, PFKFB3, PKM2 and LDHA in SCs were measured by western blot. n = 3 independent biological experiments. ( F ). Immunofluorescence staining and quantification of GLUT1, HK2, PFK-1, and PFKFB3 in SCs. Scale bar, 20 μm. N = 4 independent biological experiments, with n ≥ 6 fields/experiment. ( G ). Schematic depicting SYK deficiency-induced downregulation (red arrows) of glycolytic transporters, enzymes, and metabolites in SCs
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    Thermo Fisher fluorescent glucose analog 2 n 7 nitrobenz 2 oxa 1 3 diazol 4 yl amino
    SYK deficiency impairs glycolytic flux in SCs. ( A ). ECAR profiles measured by Seahorse experiments. ( B ). Quantification of basal glycolysis, glycolytic capacity, and glycolytic reserve in ECAR. n = 4 independent biological experiments. ( C ). Glucose uptake measured <t>with</t> <t>2-NBDG</t> by flow cytometry. n = 4 independent biological experiments. ( D ). Intracellular levels of glucose-6-phosphate (G6P), pyruvate and lactate were measured by chemiluminescence. n = 4 independent biological experiments. ( E ). Protein levels of GLUT1, GLUT4, HK2, PFK-1, PFKFB3, PKM2 and LDHA in SCs were measured by western blot. n = 3 independent biological experiments. ( F ). Immunofluorescence staining and quantification of GLUT1, HK2, PFK-1, and PFKFB3 in SCs. Scale bar, 20 μm. N = 4 independent biological experiments, with n ≥ 6 fields/experiment. ( G ). Schematic depicting SYK deficiency-induced downregulation (red arrows) of glycolytic transporters, enzymes, and metabolites in SCs
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    SYK deficiency impairs glycolytic flux in SCs. ( A ). ECAR profiles measured by Seahorse experiments. ( B ). Quantification of basal glycolysis, glycolytic capacity, and glycolytic reserve in ECAR. n = 4 independent biological experiments. ( C ). Glucose uptake measured <t>with</t> <t>2-NBDG</t> by flow cytometry. n = 4 independent biological experiments. ( D ). Intracellular levels of glucose-6-phosphate (G6P), pyruvate and lactate were measured by chemiluminescence. n = 4 independent biological experiments. ( E ). Protein levels of GLUT1, GLUT4, HK2, PFK-1, PFKFB3, PKM2 and LDHA in SCs were measured by western blot. n = 3 independent biological experiments. ( F ). Immunofluorescence staining and quantification of GLUT1, HK2, PFK-1, and PFKFB3 in SCs. Scale bar, 20 μm. N = 4 independent biological experiments, with n ≥ 6 fields/experiment. ( G ). Schematic depicting SYK deficiency-induced downregulation (red arrows) of glycolytic transporters, enzymes, and metabolites in SCs
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    Croda International Plc fluorescent cholesterol 22 n 7 nitrobenz 2 oxa 1 3diazol 4 yl amino 23 24 bisnor 5 cholen 3β ol
    SYK deficiency impairs glycolytic flux in SCs. ( A ). ECAR profiles measured by Seahorse experiments. ( B ). Quantification of basal glycolysis, glycolytic capacity, and glycolytic reserve in ECAR. n = 4 independent biological experiments. ( C ). Glucose uptake measured <t>with</t> <t>2-NBDG</t> by flow cytometry. n = 4 independent biological experiments. ( D ). Intracellular levels of glucose-6-phosphate (G6P), pyruvate and lactate were measured by chemiluminescence. n = 4 independent biological experiments. ( E ). Protein levels of GLUT1, GLUT4, HK2, PFK-1, PFKFB3, PKM2 and LDHA in SCs were measured by western blot. n = 3 independent biological experiments. ( F ). Immunofluorescence staining and quantification of GLUT1, HK2, PFK-1, and PFKFB3 in SCs. Scale bar, 20 μm. N = 4 independent biological experiments, with n ≥ 6 fields/experiment. ( G ). Schematic depicting SYK deficiency-induced downregulation (red arrows) of glycolytic transporters, enzymes, and metabolites in SCs
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    SYK deficiency impairs glycolytic flux in SCs. ( A ). ECAR profiles measured by Seahorse experiments. ( B ). Quantification of basal glycolysis, glycolytic capacity, and glycolytic reserve in ECAR. n = 4 independent biological experiments. ( C ). Glucose uptake measured with 2-NBDG by flow cytometry. n = 4 independent biological experiments. ( D ). Intracellular levels of glucose-6-phosphate (G6P), pyruvate and lactate were measured by chemiluminescence. n = 4 independent biological experiments. ( E ). Protein levels of GLUT1, GLUT4, HK2, PFK-1, PFKFB3, PKM2 and LDHA in SCs were measured by western blot. n = 3 independent biological experiments. ( F ). Immunofluorescence staining and quantification of GLUT1, HK2, PFK-1, and PFKFB3 in SCs. Scale bar, 20 μm. N = 4 independent biological experiments, with n ≥ 6 fields/experiment. ( G ). Schematic depicting SYK deficiency-induced downregulation (red arrows) of glycolytic transporters, enzymes, and metabolites in SCs

    Journal: Journal of Neuroinflammation

    Article Title: SYK regulates Schwann cell metabolic reprogramming to promote axonal regeneration in immune-mediated neuropathy

    doi: 10.1186/s12974-025-03624-y

    Figure Lengend Snippet: SYK deficiency impairs glycolytic flux in SCs. ( A ). ECAR profiles measured by Seahorse experiments. ( B ). Quantification of basal glycolysis, glycolytic capacity, and glycolytic reserve in ECAR. n = 4 independent biological experiments. ( C ). Glucose uptake measured with 2-NBDG by flow cytometry. n = 4 independent biological experiments. ( D ). Intracellular levels of glucose-6-phosphate (G6P), pyruvate and lactate were measured by chemiluminescence. n = 4 independent biological experiments. ( E ). Protein levels of GLUT1, GLUT4, HK2, PFK-1, PFKFB3, PKM2 and LDHA in SCs were measured by western blot. n = 3 independent biological experiments. ( F ). Immunofluorescence staining and quantification of GLUT1, HK2, PFK-1, and PFKFB3 in SCs. Scale bar, 20 μm. N = 4 independent biological experiments, with n ≥ 6 fields/experiment. ( G ). Schematic depicting SYK deficiency-induced downregulation (red arrows) of glycolytic transporters, enzymes, and metabolites in SCs

    Article Snippet: SCs were incubated with 100 μM 2-[N-(7-nitrobenz-2-oxa-1,3- diazol-4-yl)amino]−2-deoxy-D-glucose (2-NBDG) (MCE, Cat#HY-116215) for 30 min at 37 °C in the dark.

    Techniques: Flow Cytometry, Western Blot, Immunofluorescence, Staining